PPARG ELISA Kit
Quick Overview for PPARG ELISA Kit (ABIN4972171)
Target
See all PPARG ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 78 pg/mL
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Purpose
- Mouse Peroxisome Proliferator Activated Receptor Gamma (PPARg) ELISA Kit
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Analytical Method
- Quantitative
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Components
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The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
- Pre-coated 96-Well Microplate
- Standard
- Standard Diluent Buffer
- Wash Buffer
- Detection Reagent A
- Detection Reagent B
- Diluent A
- Diluent B
- TMB Substrate
- Stop Solution
- Plate Sealer
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Material not included
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- 37 °C incubator
- Multi and single channel pipettes and sterile pipette tips
- Squirt bottle or automated microplate washer
- 1.5 mL tubes
- Distilled water
- Absorbent filter papers
- 100 mL and 1 liter graduated cylinders
- Microplate reader (wavelength: 450 nm)
- ELISA Shaker
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Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA Kit
PPARG Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG Reactivity: Human Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG Reactivity: Rabbit Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG Reactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG Reactivity: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG Reactivity: Cow Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG Reactivity: Human Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
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Application Notes
- Optimal dilutions/concentrations should be determined by the end user.
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Comment
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The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
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Plate
- Pre-coated
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Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Peroxisome Proliferator Activated Receptor Gamma were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Peroxisome Proliferator Activated Receptor Gamma were tested on 3 different plates, 8 replicates in each plate.
CV (%) = (Standard Deviation / mean) x 100
Intra-Assay: CV<10%
Inter-Assay: CV<10%
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
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Expiry Date
- 6 months
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- PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))
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Alternative Name
- Peroxisome Proliferator Activated Receptor Gamma
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Pathways
- MAPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
Target See all PPARG ELISA Kits
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