FAS ELISA Kit

Catalog No. ABIN4986730

Human FAS ELISA Kit, Colorimetric assay for quantification of Human FAS.

Quick Overview for FAS ELISA Kit (ABIN4986730)

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Product Details FAS ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Analytical Method: Quantitative

Specificity: Natural and recombinant Human APO-1

Sensitivity: 31 pg/mL

Material not included:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Application Details

Application Notes: Detection Wavelength: 450 nm

Sample Volume: 20 μL

Assay Time: 3 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: APO-1

Background: Apoptosis inducing protein 1(Apo-1) also known as Fas (Fibroblast-associated), CD95, and TNFRSF6(325 amino acids, 45-48 kDa), was originally identified as a cell-surface protein that binds to monoclonal antibodies that are cytolytic for various human cell lines. Fas is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, activated lymphocytes and fibroblasts. Interaction of Apo on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. Fas-mediated death occurs much more rapidly than that triggered by TNFR1(Tumor necrosis factor receptor 1). Fas (APO-1 or CD95) is a cell-surface receptor that transduces apoptotic signals from Fasligand (FasL) (1, 2). It is a glycoprotein with a mass estimated at 43 to 48 kDa (3, 4). Fas is a member of the Tumor Necrosis Factor Receptor Superfamily (TNFRSF), and it shares a cytoplasmic motif with TNF RI, referred to as the

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity