IL1A ELISA Kit

Catalog No. ABIN5026929

Product Details IL1A ELISA Kit

Target: IL1A (Interleukin 1 alpha (IL1A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.9-250 pg/mL

Minimum Detection Limit: 3.9 pg/mL

Application: ELISA

Purpose: This immunoassay kit allows for the in vitro quantitative determination of Human IL-1Alpha concentrations in serum, plasma and other biological fluids.

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Sensitivity: < 2.34 pg/mL

Components: plate, standard, diluent, antibodies

Material not included: pipettes, tubes, reader, incubator

Application Details

Sample Volume: 100 μL

Assay Time: 3 - 4 h

Plate: Pre-coated

Protocol: This This assay employs an antibody specific for anti-human IL-1Alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1Alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-humanIL-1Alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1Alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Restrictions: For Research Use only

Handling

Precaution of Use: protect your eyes

Storage: 4 °C

Target Details for IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Alternative Name: IL-1alpha (Interleukin 1 Alpha) (IL1A Products)

Synonyms: IL-1A ELISA Kit, IL1 ELISA Kit, IL1-ALPHA ELISA Kit, IL1F1 ELISA Kit, IL-6 ELISA Kit, Il-1a ELISA Kit, IL-1 alpha ELISA Kit, IL-1alpha ELISA Kit, L1A ELISA Kit, interleukin 1 alpha ELISA Kit, interleukin 6 ELISA Kit, IL1A ELISA Kit, IL6 ELISA Kit, Il1a ELISA Kit

Pathways: NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints