IL12 ELISA Kit

Catalog No. ABIN5067892

This Colorimetric ELISA kit is designed for the quantitative measurement of Human IL12.

Quick Overview for IL12 ELISA Kit (ABIN5067892)

Target: IL12 (Interleukin 12 (IL12))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Urine, Plasma, Cell Culture Supernatant, Serum

Product Details IL12 ELISA Kit

Purpose: For the quantitative determination of human interleukin 12 IL-12) concentrations in cell culture supernates, serum, plasma, and urine. Recognizes native and recombinant human Il-12

Analytical Method: Quantitative

Cross-Reactivity (Details): Calculated cross reactivity: Hu

Sensitivity: 5 pg/mL

Characteristics: Interleukin 12 (IL-12), ELISA Kit (Human) (IL-12, Cytotoxic Lymphocyte Maturation Factor 1, CTL Maturation Factor, TcMF, CLMF p35, Natural Killer Cell Stimulatory Factor 1, NFSK1, p35, T cell Stimulating Factor, TSF)

Components:

• IL-12 Microplate: 96 well polystyrene microplate (12 strips of 8 wells) coated with a murine monoclonal antibody against IL-12.
• IL-12 Conjugate: 21ml of polyclonal antibody against IL-12 conjugated to horseradish peroxidase, with preservative.
• IL-12 Standard: 2.5ng of recombinant human IL-12 in a buffered protein base with preservative, lyophilized.
• Assay Diluent R 6ml of a buffered protein base with preservative. For use with cell culture supernate/serum/plasma samples.
• Assay Diluent R 6ml of a buffered protein base with preservative. For use with urine samples.
• Calibrator Diluent R Concentrate (5X): 21ml of a buffered protein base with preservative.
• Wash Buffer Concentrate: 21ml of a 25-fold concentrated solution of buffered surfactant with preservative.
• Color Reagent A: 12.5ml of stabilized hydrogen peroxide.
• Color Reagent B: 12.5ml of stabilized chromogen (tetramethylbenzidine).
• Stop Solution: 6ml of 2 N sulfuric acid.

Application Details

Plate: Pre-coated

Protocol: Principle of the Assay:

• This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL- 12 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody specific for IL-12 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-12 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: 4°C/-20°C

Target Details for IL12

Target: IL12 (Interleukin 12 (IL12))

Alternative Name: Interleukin 12 (IL-12)

Pathways: JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome