Insulin ELISA Kit

Catalog No. ABIN5520038

The Rat Insulin ELISA Kit (ABIN5520038) is a Colorimetric ELISA Kit designed to quantify Rat Insulin. This ELISA Kit has been cited in 5 publications.

Quick Overview for Insulin ELISA Kit (ABIN5520038)

Target: Insulin (INS)

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.125 pg/mL - 5000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Product Details Insulin ELISA Kit

Minimum Detection Limit: 78.125 pg/mL

Purpose: Rat INS (Insulin) ELISA Kit

Analytical Method: Quantitative

Specificity: Specifically binds with INS , no obvious cross reaction with other analogues.

Sensitivity: 46.875 pg/mL

Components:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• Lyophilized Biotin-labeled Antibody(Concentrated)
• Biotin-labeled Antibody
• HRP-Streptavidin Conjugate(SABC
• 100X)
• TMB Substrate
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Material not included:

• Microplate reader (wavelength: 450nm)
• 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Precision single (0.5-10μL
• 5-50μL
• 20-200μL
• 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Assay Time: 4 h

Plate: Pre-coated

Protocol: Step 1: Add 100 µL standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37 °C. Washing: Wash the plate twice without immersing. Step 2: Add 100 µL biotin-antibody working solution, seal the plate and statically incubate for 60 minutes at 37 °C. Washing: Wash the plate three times and immerse for 1 min each time. Step 3: Add 100 µL HRP-Streptavidin Conjugate (SABC) working solution, seal the plate and statically incubate for 30 minutes at 37 °C. Washing: Wash the plate five times and immerse for 1 min each time. Step 4: Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 5: Add 50 µL stop solution. Read at 450nm immediately and calculate.

Restrictions: For Research Use only

Handling

Handling Advice: Avoid direct light exposure to antibody solutions

Storage: 4 °C

Storage Comment: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Expiry Date: 12 months

References for Insulin ELISA Kit (ABIN5520038)

Ozcan, Canpolat, Bulmus, Ulker, Tektemur, Tekin, Ozcan, Serhatlioglu, Kacar, Ayar, Kelestimur: "Agomelatine pretreatment prevents development of hyperglycemia and hypoinsulinemia in streptozotocin-induced diabetes in mice." in: Fundamental & clinical pharmacology, Vol. 33, Issue 2, pp. 170-180, (2019) (PubMed).

Pei, Yao, Jiang, Qiu, Wang, Yang, Gao, Wang, Yang, Liu, Liu, Jia, Tao, Wei, Sun: "Inorganic arsenic induces pyroptosis and pancreatic β cells dysfunction through stimulating the IRE1α/TNF-α pathway and protective effect of taurine." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 125, pp. 392-402, (2019) (PubMed).

Liu, Wang, Ma, Wen: "Hydroxytyrosol Improves Obesity and Insulin Resistance by Modulating Gut Microbiota in High-Fat Diet-Induced Obese Mice." in: Frontiers in microbiology, Vol. 10, pp. 390, (2019) (PubMed).

Chin, Ng, Ng: "Moringa oleifera standardised aqueous leaf extract-loaded hydrocolloid film dressing: in vivo dermal safety and wound healing evaluation in STZ/HFD diabetic rat model." in: Drug delivery and translational research, (2018) (PubMed).

Qasem, Noordin, Arya, Alsalahi, Jayash: "Evaluation of the glycemic effect of Ceratonia siliqua pods (Carob) on a streptozotocin-nicotinamide induced diabetic rat model." in: PeerJ, Vol. 6, pp. e4788, (2018) (PubMed).

Target Details for Insulin

Target: Insulin (INS)

Alternative Name: INS

Background: Insulin, INS

UniProt: P01322

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation