HMGB1 ELISA Kit

Catalog No. ABIN5523372

Mouse HMGB1 ELISA Kit Colorimetric assay for quantification of Mouse HMGB1.

Quick Overview for HMGB1 ELISA Kit (ABIN5523372)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.625 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details HMGB1 ELISA Kit

Minimum Detection Limit: 15.625 pg/mL

Purpose: For quantitative detection of Hmgb1 in serum, plasma, tissue homogenates.

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Hmgb1. No significant cross-reactivity or interference between Hmgb1 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between Hmgb1 and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 9.375 pg/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

Target Details for HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: Hmgb1

Background: Hmgb1/HMG-1/Amphoterin/high mobility group box 1/High mobility group protein 1/high-mobility group(nonhistone chromosomal) protein 1/high-mobility group box 1/HMG-1/HMG1DKFZp686A04236/HMG3/SBP-1/Sulfoglucuronyl carbohydrate binding protein

UniProt: P63158

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome