IL-6 ELISA Kit

Catalog No. ABIN5523461

This Mouse IL-6 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse IL-6. This ELISA Kit has been cited in1 publication.

Quick Overview for IL-6 ELISA Kit (ABIN5523461)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.625 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details IL-6 ELISA Kit

Minimum Detection Limit: 15.625 pg/mL

Purpose: For quantitative detection of IL-6 in serum, plasma, tissue homogenates.

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of IL-6. No significant cross-reactivity or interference between IL-6 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between IL-6 and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 9.375 pg/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

References for IL-6 ELISA Kit (ABIN5523461)

Twayana, Chaudhari, Ravanan: "Prolonged lipopolysaccharide exposure induces transient immunosuppression in BV2 microglia." in: Journal of cellular physiology, Vol. 234, Issue 2, pp. 1889-1903, (2019) (PubMed).

Target Details for IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL-6

Background: IL-6(Interleukin 6)/IL6/BSF2/HGF/HSF/IFNB2/CDF

UniProt: P08505

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome