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ULK1 ELISA Kit

ULK1 Reactivity: Human, Mouse pSer556 Colorimetric Sandwich ELISA Cell Culture Lysate
Catalog No. ABIN5526681
  • Target See all ULK1 ELISA Kits
    ULK1 (Unc-51 Like Kinase 1 (ULK1))
    Binding Specificity
    pSer556, total
    Reactivity
    Human, Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human and Mouse Phospho-ULK1 (S556) and Total ULK1 ELISA Kit. This assay semi-quantitatively measures ULK1 phosphorylated at Serine-556 as well as total ULK1 in cell lysate samples.
    Sample Type
    Cell Culture Lysate
    Analytical Method
    Semi-Quantitative
    Specificity
    This ELISA kit recognizes Human and Mouse ULK1 phosphorylated at site Serine-556 as well as total ULK1.
    Characteristics
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Expiry Date
    6 months
  • Target See all ULK1 ELISA Kits
    ULK1 (Unc-51 Like Kinase 1 (ULK1))
    Alternative Name
    ULK1 (ULK1 Products)
    Synonyms
    ATG1 ELISA Kit, ATG1A ELISA Kit, UNC51 ELISA Kit, Unc51.1 ELISA Kit, hATG1 ELISA Kit, si:dkey-11o18.7 ELISA Kit, ulk1 ELISA Kit, ULK1 ELISA Kit, AU041434 ELISA Kit, mKIAA0722 ELISA Kit, zgc:195008 ELISA Kit, unc-51 like autophagy activating kinase 1 ELISA Kit, unc-51 like kinase 1 ELISA Kit, unc-51 like autophagy activating kinase 1a ELISA Kit, ULK1 ELISA Kit, Ulk1 ELISA Kit, ulk1b ELISA Kit, ulk1a ELISA Kit
    Gene ID
    8408
    UniProt
    O75385, O70405
    Pathways
    Regulation of Cell Size, Autophagy
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