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IL12 CLIA Kit

Human IL12 ELISA Kit Chemiluminescent assay for quantification of Human IL12.
Catalog No. ABIN5648379

Quick Overview for IL12 CLIA Kit (ABIN5648379)

Target

See all IL12 CLIA Kits
IL12 (Interleukin 12 (IL12))

Reactivity

  • 18
  • 10
  • 6
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Human

Detection Method

Chemiluminescent

Method Type

Sandwich ELISA

Detection Range

8 pg/mL - 500 pg/mL

Application

ELISA

Sample Type

Cell Culture Supernatant, Cell Lysate, Serum
  • Minimum Detection Limit

    8 pg/mL

    Analytical Method

    Quantitative

    Specificity

    The antigens listed below were tested at 50 ng/mL and exhibited 100 % cross reactivity. • Human: IL-12p40, IL-12p80 The antigens listed below were tested at 50 ng/mL and did not exhibit significant cross reactivity or interference. • Human: IFN-gamma, IL-10, IL-16 (121 aa), IL-16 (130 aa), IL-17A, IL-17B, IL-17D, IL-17E, IL-17F • Murine: IFN-gamma, IL-10, IL-12, IL-12p40 • Rat: IFN-gamma

    Characteristics

    Luminescent detection of samples
    - Increased sensitivity for protein concentrations from pg/mL to ng/mL
    - Light signal intensity lasting up to 30 minutes
    - No hazardous chemicals
    - Easy to use with cell lysates, supernatants, and other samples

    Components

    12x8 Well Capture Antibody Coated Microstrips, Recombinant Protein Standard, Biotin-Conjugated Detection Antibody, 400x Streptavidin, 10x Wash Buffer, Assay Diluent, Enhancer Solution, Peroxide, Adhesive Plate Seals, Technical Manual
  • Plate

    Pre-coated

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    4°C/6 Months
  • Target See all IL12 CLIA Kits

    IL12 (Interleukin 12 (IL12))

    Alternative Name

    IL-12

    Background

    Synonyms: Interleukin-12 subunit alpha/beta, Cytotoxic lymphocyte maturation factor, NK cell stimulatory factor

    NCBI Gene Symbol: IL12A/B

    Gene ID

    35923593

    UniProt

    P29459, P29460

    Pathways

    JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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