Angiotensin II ELISA Kit

Catalog No. ABIN5651797

Product Details Angiotensin II ELISA Kit

Target: Angiotensin II (Ang II)

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 24.69 pg/mL - 2000 pg/mL

Minimum Detection Limit: 24.69 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Angiotensin II (AngII). No significant cross-reactivity or interference between Angiotensin II (AngII) and analogues was observed.

Sensitivity: 9.22 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 h

Plate: Pre-coated

Protocol: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Angiotensin II (AngII) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Angiotensin II (AngII) and unlabeled Angiotensin II (AngII) (Standards or samples) with the pre-coated antibody specific to Angiotensin II (AngII). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Angiotensin II (AngII) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Angiotensin II (AngII) in the sample.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin II (AngII) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensin II (AngII) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Angiotensin II

Target: Angiotensin II (Ang II)

Alternative Name: Angiotensin II (Ang II Products)

Synonyms: ANHU ELISA Kit, SERPINA8 ELISA Kit, ANRT ELISA Kit, Ang ELISA Kit, AngII ELISA Kit, PAT ELISA Kit, AI265500 ELISA Kit, AngI ELISA Kit, Aogen ELISA Kit, Serpina8 ELISA Kit, angiotensinogen ELISA Kit, angiotensinogen (serpin peptidase inhibitor, clade A, member 8) ELISA Kit, AGT ELISA Kit, Agt ELISA Kit

Background:

Gene Name: Angiotensin II

Gene Aliases: Ang-II, Angiotensin-2

Pathways: JAK-STAT Signaling, ACE Inhibitor Pathway, EGFR Signaling Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity