Annexin a1 CLIA Kit

Catalog No. ABIN5651824

Product Details Annexin a1 CLIA Kit

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 0.78 ng/mL - 200 ng/mL

Minimum Detection Limit: 0.78 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Annexin A1 (ANXA1). No significant cross-reactivity or interference between Annexin A1 (ANXA1) and analogues was observed.

Sensitivity: 0.12 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Annexin A1 (ANXA1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Annexin A1 (ANXA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Annexin A1 (ANXA1) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Annexin A1 (ANXA1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Annexin A1 (ANXA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Alternative Name: Annexin A1 (ANXA1 Products)

Synonyms: anx1a CLIA Kit, anxa1 CLIA Kit, wu:fa01d11 CLIA Kit, wu:fa05d11 CLIA Kit, wu:fk69h01 CLIA Kit, MGC64363 CLIA Kit, anx1 CLIA Kit, lpc1 CLIA Kit, MGC89164 CLIA Kit, ANXA1 CLIA Kit, ANX1 CLIA Kit, LPC1 CLIA Kit, p35 CLIA Kit, Anx-1 CLIA Kit, Anx-A1 CLIA Kit, C430014K04Rik CLIA Kit, Lpc-1 CLIA Kit, Lpc1 CLIA Kit, Anx1 CLIA Kit, annexin A1a CLIA Kit, annexin A1 CLIA Kit, annexin A1 L homeolog CLIA Kit, Annexin A1 CLIA Kit, anxa1a CLIA Kit, anxa1 CLIA Kit, anxa1.L CLIA Kit, ANXA1 CLIA Kit, LOC100335033 CLIA Kit, Anxa1 CLIA Kit

Background:

Gene Name: Annexin A1

Gene Aliases: ANX-A1, ANX1, LPC1, Lipocortin I, Chromobindin-9, Calpactin II, Phospholipase A2 inhibitory protein

Gene ID: 301

UniProt: P04083

Pathways: Hormone Transport