SERPING1 CLIA Kit

Catalog No. ABIN5653189

Product Details SERPING1 CLIA Kit

Target: SERPING1 (Serpin Peptidase Inhibitor, Clade G (C1 Inhibitor), Member 1 (SERPING1))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 0.14 ng/mL - 100 ng/mL

Minimum Detection Limit: 0.14 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Complement 1 Inhibitor (C1INH). No significant cross-reactivity or interference between Complement 1 Inhibitor (C1INH) and analogues was observed.

Sensitivity: 0.06 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Complement 1 Inhibitor (C1INH). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Complement 1 Inhibitor (C1INH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Complement 1 Inhibitor (C1INH) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement 1 Inhibitor (C1INH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement 1 Inhibitor (C1INH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: SERPING1 (Serpin Peptidase Inhibitor, Clade G (C1 Inhibitor), Member 1 (SERPING1))

Alternative Name: Complement 1 Inhibitor (SERPING1 Products)

Synonyms: fb64f02 CLIA Kit, im:7159527 CLIA Kit, si:dkeyp-110g5.2 CLIA Kit, wu:fb60c09 CLIA Kit, wu:fb62a08 CLIA Kit, wu:fb64f02 CLIA Kit, LOC100136072 CLIA Kit, C1-INH CLIA Kit, C1INH CLIA Kit, C1nh CLIA Kit, C1IN CLIA Kit, C1NH CLIA Kit, HAE1 CLIA Kit, HAE2 CLIA Kit, serpin peptidase inhibitor, clade G (C1 inhibitor), member 1 CLIA Kit, C1 inhibitor CLIA Kit, serpin family G member 1 CLIA Kit, serine (or cysteine) peptidase inhibitor, clade G, member 1 CLIA Kit, serping1 CLIA Kit, LOC100136072 CLIA Kit, SERPING1 CLIA Kit, Serping1 CLIA Kit

Background:

Gene Name: Complement 1 Inhibitor

Gene Aliases: SERPING1, C1IN, C1-INH, C1NH, HAE1, HAE2, Serpin Peptidase Inhibitor Clade G Member 1, Angioedema Hereditary, C1 Inhibitor, C1 Esterase Inhibitor

Gene ID: 710

UniProt: P05155

Pathways: Complement System