CDK2 ELISA Kit

Catalog No. ABIN5653420

Product Details CDK2 ELISA Kit

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Cyclin Dependent Kinase 2 (CDK2). No significant cross-reactivity or interference between Cyclin Dependent Kinase 2 (CDK2) and analogues was observed.

Sensitivity: 0.133 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin Dependent Kinase 2 (CDK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclin Dependent Kinase 2 (CDK2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclin Dependent Kinase 2 (CDK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclin Dependent Kinase 2 (CDK2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyclin Dependent Kinase 2 (CDK2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyclin Dependent Kinase 2 (CDK2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CDK2

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Alternative Name: Cyclin Dependent Kinase 2 (CDK2 Products)

Synonyms: p33(CDK2) ELISA Kit, A630093N05Rik ELISA Kit, wu:fa10c02 ELISA Kit, zgc:56598 ELISA Kit, zgc:77684 ELISA Kit, eg1 ELISA Kit, CDKN2 ELISA Kit, cdk2L ELISA Kit, cyclin dependent kinase 2 ELISA Kit, cyclin-dependent kinase 2 ELISA Kit, cyclin-dependent kinase 2 S homeolog ELISA Kit, CDK2 ELISA Kit, Cdk2 ELISA Kit, cdk2 ELISA Kit, cdk2.S ELISA Kit

Background:

Gene Name: Cyclin Dependent Kinase 2

Gene Aliases: CDKN2, p33(CDK2), Cell division protein kinase 2, p33 protein kinase

UniProt: Q63699

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA