FAS CLIA Kit

Catalog No. ABIN5654035

The Mouse FAS ELISA Kit (ABIN5654035) is a Chemiluminescent ELISA Kit designed to quantify Mouse FAS.

Quick Overview for FAS CLIA Kit (ABIN5654035)

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivity: Mouse

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 4.12 pg/mL - 3000 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Plasma, Serum, Tissue Homogenate

Product Details FAS CLIA Kit

Minimum Detection Limit: 4.12 pg/mL

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Factor Related Apoptosis (FAS). No significant cross-reactivity or interference between Factor Related Apoptosis (FAS) and analogues was observed.

Sensitivity: 1.58 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Factor Related Apoptosis (FAS). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Factor Related Apoptosis (FAS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Factor Related Apoptosis (FAS) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Factor Related Apoptosis (FAS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Factor Related Apoptosis (FAS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: Factor Related Apoptosis

Background:

Gene Name: Factor Related Apoptosis

Gene Aliases: CD95, ALPS1A, ALPS1-A, APO1, APT1, FAS1, FASTM, TNFRSF6, Fas Receptor, TNF Receptor Superfamily Member 6, Tumor Necrosis Factor Receptor Superfamily Member 6

UniProt: P25446

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity