GCK CLIA Kit

Catalog No. ABIN5654607

Product Details GCK CLIA Kit

Target: GCK (Glucokinase (Hexokinase 4) (GCK))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 0.59 ng/mL - 150 ng/mL

Minimum Detection Limit: 0.59 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Glucokinase (GCK). No significant cross-reactivity or interference between Glucokinase (GCK) and analogues was observed.

Sensitivity: 0.08 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Glucokinase (GCK). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Glucokinase (GCK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Glucokinase (GCK) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucokinase (GCK) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucokinase (GCK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: GCK (Glucokinase (Hexokinase 4) (GCK))

Alternative Name: Glucokinase (GCK Products)

Synonyms: FGQTL3 CLIA Kit, GK CLIA Kit, GLK CLIA Kit, HHF3 CLIA Kit, HK4 CLIA Kit, HKIV CLIA Kit, HXKP CLIA Kit, LGLK CLIA Kit, MODY2 CLIA Kit, Gk CLIA Kit, Gls006 CLIA Kit, GCK CLIA Kit, BA4487 CLIA Kit, glk CLIA Kit, hk4 CLIA Kit, hhf3 CLIA Kit, hkiv CLIA Kit, hxkp CLIA Kit, gck-A CLIA Kit, mody2 CLIA Kit, glucokinase CLIA Kit, GLUKA CLIA Kit, RNGK2 CLIA Kit, glucokinase CLIA Kit, glucokinase (hexokinase 4) L homeolog CLIA Kit, glucokinase (hexokinase 4) CLIA Kit, GCK CLIA Kit, Gck CLIA Kit, glcK CLIA Kit, Bphyt_1025 CLIA Kit, Halhy_6548 CLIA Kit, gk CLIA Kit, gck.L CLIA Kit, gck CLIA Kit

Background:

Gene Name: Glucokinase

Gene Aliases: GK, GLK, HHF3, HK4, HKIV, HXKP, MODY2, Hexokinase-D, Hexokinase 4, Maturity Onset Diabetes Of The Young 2

Pathways: MAPK Signaling, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process