GCK ELISA Kit

Catalog No. ABIN5654610

Product Details GCK ELISA Kit

Target: GCK (Glucokinase (Hexokinase 4) (GCK))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.781 ng/mL - 50 ng/mL

Minimum Detection Limit: 0.781 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Glucokinase (GCK). No significant cross-reactivity or interference between Glucokinase (GCK) and analogues was observed.

Sensitivity: 0.24 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucokinase (GCK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucokinase (GCK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucokinase (GCK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucokinase (GCK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucokinase (GCK) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucokinase (GCK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for GCK

Target: GCK (Glucokinase (Hexokinase 4) (GCK))

Alternative Name: Glucokinase (GCK Products)

Synonyms: FGQTL3 ELISA Kit, GK ELISA Kit, GLK ELISA Kit, HHF3 ELISA Kit, HK4 ELISA Kit, HKIV ELISA Kit, HXKP ELISA Kit, LGLK ELISA Kit, MODY2 ELISA Kit, Gk ELISA Kit, Gls006 ELISA Kit, GCK ELISA Kit, BA4487 ELISA Kit, glk ELISA Kit, hk4 ELISA Kit, hhf3 ELISA Kit, hkiv ELISA Kit, hxkp ELISA Kit, gck-A ELISA Kit, mody2 ELISA Kit, glucokinase ELISA Kit, GLUKA ELISA Kit, RNGK2 ELISA Kit, glucokinase ELISA Kit, glucokinase (hexokinase 4) L homeolog ELISA Kit, glucokinase (hexokinase 4) ELISA Kit, GCK ELISA Kit, Gck ELISA Kit, glcK ELISA Kit, Bphyt_1025 ELISA Kit, Halhy_6548 ELISA Kit, gk ELISA Kit, gck.L ELISA Kit, gck ELISA Kit

Background:

Gene Name: Glucokinase

Gene Aliases: GK, GLK, HHF3, HK4, HKIV, HXKP, MODY2, Hexokinase-D, Hexokinase 4, Maturity Onset Diabetes Of The Young 2

Gene ID: 24385

UniProt: P17712

Pathways: MAPK Signaling, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process