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IGF1 CLIA Kit

IGF1 Reactivity: Mouse Chemiluminescent Sandwich ELISA 312.5 pg/mL - 20000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Catalog No. ABIN5655398
  • Target See all IGF1 CLIA Kits
    IGF1 (Insulin-Like Growth Factor 1 (IGF1))
    Reactivity
    • 8
    • 7
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Chemiluminescent
    Method Type
    Sandwich ELISA
    Detection Range
    312.5 pg/mL - 20000 pg/mL
    Minimum Detection Limit
    312.5 pg/mL
    Application
    ELISA
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor 1 (IGF1). No significant cross-reactivity or interference between Insulin Like Growth Factor 1 (IGF1) and analogues was observed.
    Sensitivity
    13.3 pg/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    2 - 3 h
    Plate
    Pre-coated
    Protocol
    The microplate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 1 (IGF1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor 1 (IGF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Insulin Like Growth Factor 1 (IGF1) level in the sample or standard.,
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor 1 (IGF1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor 1 (IGF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all IGF1 CLIA Kits
    IGF1 (Insulin-Like Growth Factor 1 (IGF1))
    Alternative Name
    Insulin Like Growth Factor 1 (IGF1 Products)
    Synonyms
    IGF-I CLIA Kit, IGF1A CLIA Kit, IGFI CLIA Kit, C730016P09Rik CLIA Kit, Igf-1 CLIA Kit, Igf-I CLIA Kit, Npt2B CLIA Kit, IGF-1 CLIA Kit, IGFIA CLIA Kit, IGF-IB CLIA Kit, igf1 CLIA Kit, IGF-1L CLIA Kit, IGF-1a CLIA Kit, IGF1 CLIA Kit, igf-1 CLIA Kit, igf1-A CLIA Kit, igf1.S CLIA Kit, xigf1 CLIA Kit, insulin like growth factor 1 CLIA Kit, insulin-like growth factor 1 CLIA Kit, insulin-like growth factor I CLIA Kit, insulin like growth factor 1 L homeolog CLIA Kit, IGF1 CLIA Kit, Igf1 CLIA Kit, LOC100136741 CLIA Kit, igf1 CLIA Kit, LOC678666 CLIA Kit, igf1.L CLIA Kit, LOC104911603 CLIA Kit
    Background

    Gene Name: Insulin Like Growth Factor 1

    Gene Aliases: IGFI, IGF1A, IBP1, MGF, Somatomedin C, Mechano Growth Gactor

    Gene ID
    16000
    UniProt
    Q8CAR0
    Pathways
    RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation
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