Insulin Receptor ELISA Kit

Catalog No. ABIN5655460

The Human Insulin Receptor ELISA Kit (ABIN5655460) is a Colorimetric ELISA Kit designed to quantify Human Insulin Receptor.

Quick Overview for Insulin Receptor ELISA Kit (ABIN5655460)

Target: Insulin Receptor (INSR)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.12 ng/mL - 200 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details Insulin Receptor ELISA Kit

Minimum Detection Limit: 3.12 ng/mL

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Insulin Receptor (ISR). No significant cross-reactivity or interference between Insulin Receptor (ISR) and analogues was observed.

Sensitivity: 1.37 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to Insulin Receptor (ISR) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled Insulin Receptor (ISR) and unlabeled Insulin Receptor (ISR) (Standards or samples) with the pre-coated antibody specific to Insulin Receptor (ISR). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of Insulin Receptor (ISR) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Insulin Receptor (ISR) in the sample.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Receptor (ISR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Receptor (ISR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Insulin Receptor

Target: Insulin Receptor (INSR)

Alternative Name: Insulin Receptor

Background:

Gene Name: Insulin Receptor

Gene Aliases: CD220, INSR, HHF5, IR

Gene ID: 3643

UniProt: P06213

Pathways: NF-kappaB Signaling, RTK Signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity