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AGLU ELISA Kit

AGLU Reactivity: Human Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
Catalog No. ABIN5656632
  • Target See all AGLU ELISA Kits
    AGLU (alpha-Glucosidase (AGLU))
    Reactivity
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    1.56 ng/mL - 100 ng/mL
    Minimum Detection Limit
    1.56 ng/mL
    Application
    ELISA
    Sample Type
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Maltase Glucoamylase, Intestinal (MGA). No significant cross-reactivity or interference between Maltase Glucoamylase, Intestinal (MGA) and analogues was observed.
    Sensitivity
    0.54 ng/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Maltase Glucoamylase, Intestinal (MGA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Maltase Glucoamylase, Intestinal (MGA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Maltase Glucoamylase, Intestinal (MGA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Maltase Glucoamylase, Intestinal (MGA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Maltase Glucoamylase, Intestinal (MGA) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Maltase Glucoamylase, Intestinal (MGA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all AGLU ELISA Kits
    AGLU (alpha-Glucosidase (AGLU))
    Alternative Name
    Maltase Glucoamylase, Intestinal (AGLU Products)
    Synonyms
    MG ELISA Kit, MGA ELISA Kit, SPAC56F8.01 ELISA Kit, SPAC922.02c ELISA Kit, CG11909 ELISA Kit, CT33098 ELISA Kit, Dmel\\CG11909 ELISA Kit, GB19017 ELISA Kit, 6030407P20Rik ELISA Kit, maltase-glucoamylase ELISA Kit, alpha-glucosidase (predicted) ELISA Kit, alpha-glucosidase ELISA Kit, glucosidase, alpha, acid ELISA Kit, target of brain insulin ELISA Kit, sucrase-isomaltase ELISA Kit, MGAM ELISA Kit, SPAC30D11.01c ELISA Kit, LMOf2365_0194 ELISA Kit, SPAC1039.11c ELISA Kit, Gaa ELISA Kit, tobi ELISA Kit, SI ELISA Kit, Hbg3 ELISA Kit, Mgam ELISA Kit
    Background

    Gene Name: Maltase Glucoamylase, Intestinal

    Gene Aliases: MGAM, Glucan 1,4-Alpha-Glucosidase

    Gene ID
    8972
    UniProt
    O43451
    Pathways
    Cellular Glucan Metabolic Process
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