Nephrin CLIA Kit

Catalog No. ABIN5657205

Product Details Nephrin CLIA Kit

Target: Nephrin (NPHS1) (Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1))

Reactivity: Mouse

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 156.25 pg/mL - 40000 pg/mL

Minimum Detection Limit: 156.25 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Tissue Homogenate, Urine

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Nephrin (NPHN). No significant cross-reactivity or interference between Nephrin (NPHN) and analogues was observed.

Sensitivity: 18.5 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Nephrin (NPHN). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Nephrin (NPHN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Nephrin (NPHN) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nephrin (NPHN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nephrin (NPHN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: Nephrin (NPHS1) (Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1))

Alternative Name: Nephrin (NPHS1 Products)

Synonyms: NPHS1 CLIA Kit, nphs1l CLIA Kit, nphs1 CLIA Kit, CNF CLIA Kit, NPHN CLIA Kit, nephrin CLIA Kit, NephrinB CLIA Kit, NPHS1, nephrin CLIA Kit, nephrosis 1, congenital, Finnish type (nephrin) CLIA Kit, NPHS1 nephrin S homeolog CLIA Kit, nephrin CLIA Kit, NPHS1 nephrin CLIA Kit, nephrosis 1, nephrin CLIA Kit, NPHS1 CLIA Kit, nphs1 CLIA Kit, nphs1.S CLIA Kit, LOC5579127 CLIA Kit, Nphs1 CLIA Kit

Background:

Gene Name: Nephrin

Gene Aliases: NPHS1, CNF, Nephrosis 1, Congenital Finnish Type, Renal glomerulus-specific cell adhesion receptor

Gene ID: 54631

UniProt: Q9QZS7

Pathways: Regulation of Actin Filament Polymerization, Skeletal Muscle Fiber Development