PARP1 CLIA Kit

Catalog No. ABIN5657973

Product Details PARP1 CLIA Kit

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 3.12 ng/mL - 200 ng/mL

Minimum Detection Limit: 3.12 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Poly ADP Ribose Polymerase (PARP). No significant cross-reactivity or interference between Poly ADP Ribose Polymerase (PARP) and analogues was observed.

Sensitivity: 0.11 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Poly ADP Ribose Polymerase (PARP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Poly ADP Ribose Polymerase (PARP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Poly ADP Ribose Polymerase (PARP) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Poly ADP Ribose Polymerase (PARP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Poly ADP Ribose Polymerase (PARP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: Poly ADP Ribose Polymerase (PARP1 Products)

Synonyms: ADPRT CLIA Kit, ADPRT 1 CLIA Kit, ADPRT1 CLIA Kit, ARTD1 CLIA Kit, PARP CLIA Kit, PARP-1 CLIA Kit, PPOL CLIA Kit, pADPRT-1 CLIA Kit, Adprt CLIA Kit, Parp-1 CLIA Kit, 5830444G22Rik CLIA Kit, AI893648 CLIA Kit, Adprp CLIA Kit, Adprt1 CLIA Kit, C80510 CLIA Kit, parp-1 CLIA Kit, sPARP-1 CLIA Kit, BEST:LD21673 CLIA Kit, CG17685 CLIA Kit, CG17696 CLIA Kit, CG17718 CLIA Kit, CG40411 CLIA Kit, D.PARP CLIA Kit, Dm.pARTa CLIA Kit, Dmel\\CG40411 CLIA Kit, LD21673.3prime CLIA Kit, PARP1 CLIA Kit, dPARP CLIA Kit, parp CLIA Kit, adprt1 CLIA Kit, padprt-1 CLIA Kit, ppol CLIA Kit, si:dkey-206f10.3 CLIA Kit, wu:fc60f12 CLIA Kit, zgc:110092 CLIA Kit, poly(ADP-ribose) polymerase 1 CLIA Kit, poly (ADP-ribose) polymerase 1 CLIA Kit, poly (ADP-ribose) polymerase family, member 1 CLIA Kit, Poly-(ADP-ribose) polymerase CLIA Kit, poly(ADP-ribose) polymerase 1 L homeolog CLIA Kit, PARP1 CLIA Kit, Parp1 CLIA Kit, Parp CLIA Kit, parp1.L CLIA Kit, parp1 CLIA Kit

Background:

Gene Name: Poly ADP Ribose Polymerase

Gene Aliases: PARP1, ADPRT, ADPRT1, PPOL, pADPRT-1, ADP-ribosyltransferase diphtheria toxin-like 1, NAD(+) ADP-ribosyltransferase 1

Gene ID: 142

UniProt: P09874

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location