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SHP1 ELISA Kit

PTPN6 Reactivity: Human Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Tissue Homogenate
Catalog No. ABIN5658263
  • Target See all SHP1 (PTPN6) ELISA Kits
    SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))
    Reactivity
    • 9
    • 5
    • 2
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.312 ng/mL - 20 ng/mL
    Minimum Detection Limit
    0.312 ng/mL
    Application
    ELISA
    Sample Type
    Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Protein Tyrosine Phosphatase, Non Receptor Type 6 (PTPN6). No significant cross-reactivity or interference between Protein Tyrosine Phosphatase, Non Receptor Type 6 (PTPN6) and analogues was observed.
    Sensitivity
    0.105 ng/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Tyrosine Phosphatase, Non Receptor Type 6 (PTPN6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Tyrosine Phosphatase, Non Receptor Type 6 (PTPN6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Tyrosine Phosphatase, Non Receptor Type 6 (PTPN6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Tyrosine Phosphatase, Non Receptor Type 6 (PTPN6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Tyrosine Phosphatase, Non Receptor Type 6 (PTPN6) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Tyrosine Phosphatase, Non Receptor Type 6 (PTPN6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all SHP1 (PTPN6) ELISA Kits
    SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))
    Alternative Name
    Protein Tyrosine Phosphatase, Non Receptor Type 6 (PTPN6 Products)
    Synonyms
    HCPH ELISA Kit, HPTP1C ELISA Kit, PTP-1C ELISA Kit, SH-PTP1 ELISA Kit, SHP-1 ELISA Kit, SHP-1L ELISA Kit, SHP1 ELISA Kit, DRPLA ELISA Kit, Hcph ELISA Kit, Ptp1C ELISA Kit, me ELISA Kit, motheaten ELISA Kit, Ptph6 ELISA Kit, Shp-1 ELISA Kit, fj22f05 ELISA Kit, wu:fj22f05 ELISA Kit, zgc:55501 ELISA Kit, ptpn6 ELISA Kit, protein tyrosine phosphatase, non-receptor type 6 ELISA Kit, protein tyrosine phosphatase, non-receptor type 6 L homeolog ELISA Kit, PTPN6 ELISA Kit, Ptpn6 ELISA Kit, ptpn6 ELISA Kit, ptpn6.L ELISA Kit
    Background

    Gene Name: Protein Tyrosine Phosphatase, Non Receptor Type 6

    Gene Aliases: HCP, HCPH, HPTP1C, SH-PTP1, SHP-1L, SHP1, Hematopoietic cell protein-tyrosine phosphatase, Protein-tyrosine phosphatase 1C, Protein-tyrosine phosphatase SHP-1

    Gene ID
    5777
    UniProt
    P29350
    Pathways
    JAK-STAT Signaling, TCR Signaling, TLR Signaling, Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, BCR Signaling
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