SEMA3A CLIA Kit

Catalog No. ABIN5658691

Product Details SEMA3A CLIA Kit

Target: SEMA3A (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 156.25 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156.25 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Semaphorin 3A (SEMA3A). No significant cross-reactivity or interference between Semaphorin 3A (SEMA3A) and analogues was observed.

Sensitivity: 5.38 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Semaphorin 3A (SEMA3A). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Semaphorin 3A (SEMA3A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Semaphorin 3A (SEMA3A) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Semaphorin 3A (SEMA3A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Semaphorin 3A (SEMA3A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: SEMA3A (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A))

Alternative Name: Semaphorin 3A (SEMA3A Products)

Synonyms: COLL1 CLIA Kit, HH16 CLIA Kit, Hsema-I CLIA Kit, Hsema-III CLIA Kit, SEMA1 CLIA Kit, SEMAD CLIA Kit, SEMAIII CLIA Kit, SEMAL CLIA Kit, SemD CLIA Kit, coll-1 CLIA Kit, Semad CLIA Kit, COLL-1 CLIA Kit, SEMA3A CLIA Kit, Dsema3a CLIA Kit, Sema3A CLIA Kit, sema1a CLIA Kit, sema3a1 CLIA Kit, semaZ1a CLIA Kit, sema1b CLIA Kit, sema3a2 CLIA Kit, semaZ1b CLIA Kit, semaphorin 3A CLIA Kit, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A CLIA Kit, Sema-3a CLIA Kit, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Aa CLIA Kit, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Ab CLIA Kit, SEMA3A CLIA Kit, Sema3a CLIA Kit, Sema-3a CLIA Kit, sema3aa CLIA Kit, sema3ab CLIA Kit

Background:

Gene Name: Semaphorin 3A

Gene Aliases: SEMAL, Hsema-I, Hsema-III, SEMA1, SEMAD, SEMAIII, SemD, Coll-1, Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3A

Gene ID: 10371

UniProt: Q14563

Pathways: Regulation of Cell Size