SOCS1 ELISA Kit

Catalog No. ABIN5674725

Product Details SOCS1 ELISA Kit

Target: SOCS1 (Suppressor of Cytokine Signaling 1 (SOCS1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0.14 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for SOCS1

Target: SOCS1 (Suppressor of Cytokine Signaling 1 (SOCS1))

Alternative Name: Suppressors Of Cytokine Signaling 1 (SOCS1) (SOCS1 Products)

Synonyms: socs1 ELISA Kit, zgc:91868 ELISA Kit, jab ELISA Kit, cis1 ELISA Kit, ssi1 ELISA Kit, tip3 ELISA Kit, cish1 ELISA Kit, ssi-1 ELISA Kit, socs-1 ELISA Kit, SOCS1 ELISA Kit, LOC100228306 ELISA Kit, socs1b ELISA Kit, CIS1 ELISA Kit, CISH1 ELISA Kit, JAB ELISA Kit, SOCS-1 ELISA Kit, SSI-1 ELISA Kit, SSI1 ELISA Kit, TIP3 ELISA Kit, Cish1 ELISA Kit, Cish7 ELISA Kit, Socs-1 ELISA Kit, suppressor of cytokine signaling 1a ELISA Kit, suppressor of cytokine signaling 1 ELISA Kit, suppressor of cytokine signaling 1 L homeolog ELISA Kit, socs1a ELISA Kit, socs1 ELISA Kit, SOCS1 ELISA Kit, socs1.L ELISA Kit, Socs1 ELISA Kit

Background: Alternative name: CIS1, CISH1, JAB, SOCS-1, SSI-1, SSI1, TIP3, JAK-binding protein, STAT-induced STAT inhibitor 1, Tec-interacting protein 3

Gene ID: 12703

UniProt: O35716

Pathways: JAK-STAT Signaling, Interferon-gamma Pathway, TLR Signaling, Response to Growth Hormone Stimulus