APPBP2 ELISA Kit

Catalog No. ABIN5674750

Product Details APPBP2 ELISA Kit

Target: APPBP2 (Amyloid beta Precursor Protein (Cytoplasmic Tail) Binding Protein 2 (APPBP2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0115 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for APPBP2

Target: APPBP2 (Amyloid beta Precursor Protein (Cytoplasmic Tail) Binding Protein 2 (APPBP2))

Alternative Name: Amyloid Beta Precursor Protein Binding Protein 2 (APPBP2) (APPBP2 Products)

Synonyms: HS.84084 ELISA Kit, PAT1 ELISA Kit, 1300003O07Rik ELISA Kit, AI465480 ELISA Kit, zgc:76971 ELISA Kit, wu:fb78e11 ELISA Kit, wu:fc25c09 ELISA Kit, APPBP2 ELISA Kit, DKFZp459F0530 ELISA Kit, amyloid beta precursor protein binding protein 2 ELISA Kit, amyloid beta precursor protein (cytoplasmic tail) binding protein 2 ELISA Kit, amyloid beta precursor protein (cytoplasmic tail) binding protein 2 S homeolog ELISA Kit, APPBP2 ELISA Kit, Appbp2 ELISA Kit, appbp2 ELISA Kit, appbp2.S ELISA Kit

Background: Alternative name: PAT1, Protein Interacting With APP Tail 1,Cytoplasmic Tail, Amyloid protein-binding protein 2, Protein interacting with APP tail 1

Gene ID: 66884

UniProt: Q9DAX9