APBB3 ELISA Kit

Catalog No. ABIN5674812

Product Details APBB3 ELISA Kit

Target: APBB3 (Amyloid beta (A4) Precursor Protein-Binding, Family B, Member 3 (APBB3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.781 ng/mL - 50 ng/mL

Minimum Detection Limit: 0.781 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0.34 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for APBB3

Target: APBB3 (Amyloid beta (A4) Precursor Protein-Binding, Family B, Member 3 (APBB3))

Alternative Name: Amyloid Beta Precursor Protein Binding Protein B3 (APBB3) (APBB3 Products)

Synonyms: Fe65l2 ELISA Kit, Rirl2 ELISA Kit, TR2S ELISA Kit, MGC142933 ELISA Kit, FE65L2 ELISA Kit, SRA ELISA Kit, amyloid beta (A4) precursor protein-binding, family B, member 3 ELISA Kit, steroid receptor RNA activator 1 ELISA Kit, amyloid beta precursor protein binding family B member 3 ELISA Kit, Apbb3 ELISA Kit, SRA1 ELISA Kit, APBB3 ELISA Kit, apbb3 ELISA Kit

Background: Alternative name: APB-B3, SRA, FE65L2, FE65-L2, Fe65L2 isoform 484, Alzheimer's beta-amyloid interaction protein, Amyloid beta (A4) protein-binding, family B, member 3, isoform CRA_g

Gene ID: 10307

UniProt: O95704