Target See all HADHB ELISA Kits: HADHB (Hydroxyacyl-CoA Dehydrogenase/3-Ketoacyl-CoA Thiolase/enoyl-CoA Hydratase (Trifunctional Protein), beta Subunit (HADHB))
Reactivity 2 2 1 1: Human
Detection Method: Colorimetric
Method Type: Sandwich ELISA
Detection Range: 0.156 ng/mL - 10 ng/mL
Minimum Detection Limit: 0.156 ng/mL
Application: ELISA
Sample Type: Tissue Homogenate
Analytical Method: Quantitative
Sensitivity: 0053 ng/mL
Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual
Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.
Top Product: Discover our top product HADHB ELISA Kit

HADHB Reactivity: Mouse Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate

HADHB Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Tissue Homogenate

HADHB Reactivity: Human Mitochondrial Colorimetric 0.156-10 ng/mL

HADHB Reactivity: Mouse Mitochondrial Colorimetric

HADHB Reactivity: Rat Mitochondrial Colorimetric

HADHB Reactivity: Cow Mitochondrial Colorimetric

Application Notes: Optimal working dilution should be determined by the investigator.
Assay Time: 1 - 4.5 h
Plate: Pre-coated
Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.
Restrictions: For Research Use only

Target See all HADHB ELISA Kits: HADHB (Hydroxyacyl-CoA Dehydrogenase/3-Ketoacyl-CoA Thiolase/enoyl-CoA Hydratase (Trifunctional Protein), beta Subunit (HADHB))
Alternative Name: Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb) (HADHB Products)
Synonyms: ECHB ELISA Kit, MTPB ELISA Kit, TP-BETA ELISA Kit, thiolase ELISA Kit, fb14g10 ELISA Kit, wu:fb14g10 ELISA Kit, zgc:56274 ELISA Kit, 4930479F15Rik ELISA Kit, Mtpb ELISA Kit, Hadhb ELISA Kit, hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta ELISA Kit, hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta S homeolog ELISA Kit, hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), beta subunit ELISA Kit, hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit ELISA Kit, HADHB ELISA Kit, hadhb.S ELISA Kit, Hadhb ELISA Kit, hadhb ELISA Kit
Background: Alternative name: MTPB, Mitochondrial Trifunctional Protein,Beta Subunit, 3-Ketoacyl-Coenzyme A Thiolase/Enoyl-Coenzyme A Hydratase(Trifunctional Protein)Beta Subunit, Beta-ketothiolase
Gene ID: 3032
UniProt: P55084
Pathways: Monocarboxylic Acid Catabolic Process

HADHB ELISA Kit

Product Details HADHB ELISA Kit