PAX6 ELISA Kit

Catalog No. ABIN5674916

Product Details PAX6 ELISA Kit

Target: PAX6 (Paired Box 6 (PAX6))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0144 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for PAX6

Target: PAX6 (Paired Box 6 (PAX6))

Alternative Name: Paired Box Gene 6 (PAX6) (PAX6 Products)

Synonyms: pax6 ELISA Kit, an2 ELISA Kit, mgda ELISA Kit, wagr ELISA Kit, pax-6 ELISA Kit, pax6a ELISA Kit, xpax6 ELISA Kit, XLPAX6 ELISA Kit, MGC52531 ELISA Kit, PAX-6 ELISA Kit, AN ELISA Kit, AN2 ELISA Kit, D11S812E ELISA Kit, MGDA ELISA Kit, WAGR ELISA Kit, Pax-6 ELISA Kit, pax6b ELISA Kit, 1500038E17Rik ELISA Kit, AEY11 ELISA Kit, Dey ELISA Kit, Gsfaey11 ELISA Kit, Sey ELISA Kit, PAXZF-A ELISA Kit, Pax6.1 ELISA Kit, cb280 ELISA Kit, fc20e07 ELISA Kit, pax-a ELISA Kit, pax[zf-a] ELISA Kit, paxzfa ELISA Kit, wu:fc20e07 ELISA Kit, zfpax-6a ELISA Kit, zfpax-6b ELISA Kit, paired box 6 ELISA Kit, paired box 6 L homeolog ELISA Kit, paired box 6 S homeolog ELISA Kit, paired box 6a ELISA Kit, PAX6 ELISA Kit, pax6 ELISA Kit, Pax6 ELISA Kit, pax6.L ELISA Kit, pax6.S ELISA Kit, pax6a ELISA Kit

Background: Alternative name: AN, AN2, MGDA, WAGR, Oculorhombin, Aniridia,Keratitis, Aniridia type II protein

Gene ID: 5080

UniProt: P26367

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis