NPPC ELISA Kit
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- Target See all NPPC ELISA Kits
- NPPC (Natriuretic Peptide Type C (NPPC))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- Human C-type Natriuretic Peptide/CNP ELISA Kit.
- Sample Type
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Specificity
- This ELISA antibody pair recognizes Human C-type Natriuretic Peptide.
- Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Plate
- Pre-coated
- Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Expiry Date
- 6 months
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- Target See all NPPC ELISA Kits
- NPPC (Natriuretic Peptide Type C (NPPC))
- Alternative Name
- C-type Natriuretic Peptide (CNP (NPPC Products)
- Synonyms
- CNPF ELISA Kit, CNPI ELISA Kit, CNPII ELISA Kit, Cnp1 ELISA Kit, CNP ELISA Kit, lbab ELISA Kit, CNP2 ELISA Kit, CNP3 ELISA Kit, cnp ELISA Kit, nppc ELISA Kit, 2',3'-cyclic nucleotide 3' phosphodiesterase ELISA Kit, natriuretic peptide type C ELISA Kit, natriuretic peptide C ELISA Kit, natriuretic peptide C S homeolog ELISA Kit, Cnp ELISA Kit, Nppc ELISA Kit, NPPC ELISA Kit, nppc.S ELISA Kit
- Gene ID
- 4880
- UniProt
- P23582
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