Caspase 9 ELISA Kit
CASP9
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Target
See all Caspase 9 (CASP9) ELISA Kits
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Caspase 9 (CASP9)
(Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))
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Reactivity
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Human
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Detection Method
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Colorimetric
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Method Type
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Sandwich ELISA
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Application
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ELISA
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Purpose
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Human Caspase-9 ELISA Kit.
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Sample Type
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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Specificity
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This ELISA antibody pair recognizes Human Caspase-9.
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Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Plate
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Pre-coated
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Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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Restrictions
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For Research Use only
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Storage
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4 °C
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Expiry Date
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6 months
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Target
See all Caspase 9 (CASP9) ELISA Kits
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Caspase 9 (CASP9)
(Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))
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Alternative Name
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Caspase-9 (CASP9 Products)
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Synonyms
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CASP9 ELISA Kit, AI115399 ELISA Kit, APAF-3 ELISA Kit, AW493809 ELISA Kit, CASP-9 ELISA Kit, Caspase-9 ELISA Kit, ICE-LAP6 ELISA Kit, Mch6 ELISA Kit, Apaf3 ELISA Kit, Casp-9-CTD ELISA Kit, Casp9_v1 ELISA Kit, Ice-Lap6 ELISA Kit, APAF3 ELISA Kit, MCH6 ELISA Kit, PPP1R56 ELISA Kit, caspase 9 ELISA Kit, CASP9 ELISA Kit, Casp9 ELISA Kit
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Gene ID
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842
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UniProt
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P55211
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Pathways
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MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity
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