CITED2 ELISA Kit

Catalog No. ABIN5690853

Product Details CITED2 ELISA Kit

Target: CITED2 (Cbp/p300-Interacting Transactivator, with Glu/Asp-Rich Carboxy-terminal Domain, 2 (CITED2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human Cited-2 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human Cited-2.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for CITED2

Target: CITED2 (Cbp/p300-Interacting Transactivator, with Glu/Asp-Rich Carboxy-terminal Domain, 2 (CITED2))

Alternative Name: Cited-2 (CITED2 Products)

Synonyms: mrg1 ELISA Kit, p35srj ELISA Kit, zgc:103418 ELISA Kit, CITED2 ELISA Kit, cite2 ELISA Kit, ASD8 ELISA Kit, MRG-1 ELISA Kit, MRG1 ELISA Kit, P35SRJ ELISA Kit, VSD2 ELISA Kit, AI835299 ELISA Kit, ER154-like ELISA Kit, Mrg1 ELISA Kit, Msg2 ELISA Kit, Cbp/p300-interacting transactivator, with Glu/Asp rich carboxy-terminal domain, 2 ELISA Kit, Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2 ELISA Kit, Cbp/p300 interacting transactivator with Glu/Asp rich carboxy-terminal domain 2 ELISA Kit, Cbp/p300-interacting transactivator 2 ELISA Kit, Cbp/p300-interacting transactivator, with Glu/Asp rich carboxy-terminal domain, 2 L homeolog ELISA Kit, cited2 ELISA Kit, CITED2 ELISA Kit, cite2 ELISA Kit, Cited2 ELISA Kit, cited2.L ELISA Kit

Gene ID: 10370

UniProt: Q99967

Pathways: Tube Formation