ERK2 ELISA Kit

Catalog No. ABIN5706914

Product Details ERK2 ELISA Kit

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0.061 ng/mL

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual

Sample Volume: 100 µL

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for ERK2

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Alternative Name: Extracellular Signal Regulated Kinase 2 (ERK2) (MAPK1 Products)

Synonyms: ERK-1 ELISA Kit, ERK1 ELISA Kit, ERT2 ELISA Kit, HS44KDAP ELISA Kit, HUMKER1A ELISA Kit, P44ERK1 ELISA Kit, P44MAPK ELISA Kit, PRKM3 ELISA Kit, p44-ERK1 ELISA Kit, p44-MAPK ELISA Kit, Erk-1 ELISA Kit, Erk1 ELISA Kit, Ert2 ELISA Kit, Esrk1 ELISA Kit, Mnk1 ELISA Kit, Mtap2k ELISA Kit, Prkm3 ELISA Kit, p44 ELISA Kit, p44erk1 ELISA Kit, p44mapk ELISA Kit, ERK ELISA Kit, ERK2 ELISA Kit, ERT1 ELISA Kit, MAPK2 ELISA Kit, P42MAPK ELISA Kit, PRKM1 ELISA Kit, PRKM2 ELISA Kit, p38 ELISA Kit, p40 ELISA Kit, p41 ELISA Kit, p41mapk ELISA Kit, 9030612K14Rik ELISA Kit, AA407128 ELISA Kit, AU018647 ELISA Kit, C78273 ELISA Kit, Erk2 ELISA Kit, Prkm1 ELISA Kit, p42mapk ELISA Kit, zERK2 ELISA Kit, MAPK1 ELISA Kit, Xp42 ELISA Kit, erk ELISA Kit, erk2 ELISA Kit, ert1 ELISA Kit, mapk ELISA Kit, mapk1b ELISA Kit, mapk2 ELISA Kit, mpk1 ELISA Kit, prkm1 ELISA Kit, prkm2 ELISA Kit, mapk1 ELISA Kit, mapk1a ELISA Kit, xp42 ELISA Kit, ATMPK1 ELISA Kit, F14N23.9 ELISA Kit, F14N23_9 ELISA Kit, MITOGEN-ACTIVATED PROTEIN KINASE ELISA Kit, mitogen-activated protein kinase 1 ELISA Kit, MNK1 ELISA Kit, ATMPK2 ELISA Kit, MITOGEN-ACTIVATED PROTEIN KINASE HOMOLOG 2 ELISA Kit, T30E16.13 ELISA Kit, T30E16_13 ELISA Kit, mitogen-activated protein kinase homolog 2 ELISA Kit, mitogen-activated protein kinase 3 ELISA Kit, mitogen-activated protein kinase 1 ELISA Kit, mitogen activated protein kinase 1 ELISA Kit, mitogen-activated protein kinase 1 L homeolog ELISA Kit, mitogen-activated protein kinase 1 S homeolog ELISA Kit, mitogen activated protein kinase 3 ELISA Kit, mitogen-activated protein kinase homolog 2 ELISA Kit, MAPK3 ELISA Kit, Mapk3 ELISA Kit, MAPK1 ELISA Kit, Mapk1 ELISA Kit, mapk1 ELISA Kit, mapk1.L ELISA Kit, mapk1.S ELISA Kit, PfMAP1 ELISA Kit, MPK1 ELISA Kit, PVX_084965 ELISA Kit, LOC9328617 ELISA Kit, Tsp_01601 ELISA Kit, MPK2 ELISA Kit

Background: Alternative name: MAPK1, P38, P40, ERK2, ERT1, MAPK2, P42MAPK, P42-MAPK, PRKM1, PRKM2, P41, P41mapk, P41-mapk, Mitogen-Activated Protein Kinase 1, Mitogen-activated protein kinase 2

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins