NMDAR2A ELISA Kit

Catalog No. ABIN5706916

Product Details NMDAR2A ELISA Kit

Target: NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0.069 ng/mL

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual

Sample Volume: 100 µL

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for NMDAR2A

Target: NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))

Alternative Name: Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2A (GRIN2A) (GRIN2A Products)

Synonyms: NMDAR2A ELISA Kit, nr2a ELISA Kit, GRIN2A ELISA Kit, EPND ELISA Kit, GluN2A ELISA Kit, NR2A ELISA Kit, glutamate ionotropic receptor NMDA type subunit 2A ELISA Kit, glutamate receptor, ionotropic, N-methyl D-aspartate 2A L homeolog ELISA Kit, glutamate receptor ionotropic, NMDA 2A ELISA Kit, glutamate receptor, ionotropic, NMDA2A (epsilon 1) ELISA Kit, GRIN2A ELISA Kit, grin2a.L ELISA Kit, LOC100438553 ELISA Kit, Grin2a ELISA Kit

Background: Alternative name: NMDAR2A, NR2A, Glutamate [NMDA] receptor subunit epsilon-1, N-methyl D-aspartate receptor subtype 2A

Pathways: Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity