HAMA ELISA Kit

Catalog No. ABIN577098

Product Details HAMA ELISA Kit

Target: HAMA (Human Anti-Mouse Antibody (HAMA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: This assay applies an enzyme-linked immunosorbent assay (ELISA) technique. A purified mouse IgG has been pre-coated onto the wells of microplate. Standard (rabbit anti-mouse IgG) and specimens are added into the appropriate wells simultaneously with a Biotin-labelled mouse IgG, and incubated. The Human Anti-Mouse Antibody (HAMA), if present, will bind with the mouse IgG which coated on the wells and Biotin- labelled mouse IgG as well, forming a

Analytical Method: Quantitative

Sensitivity: The minimum detectable dose of HAMA was determined by adding two standard deviations to the mean optical density value of 10 zero standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose of HAMA is 3 ng/mL. S7.5(03) HAMA 10

Components: Standards: 1 set/2 vials

Application Details

Sample Volume: 50 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Preservative: Without preservative

Target Details for HAMA

Target: HAMA (Human Anti-Mouse Antibody (HAMA))

Abstract: HAMA Products

Target Type: Antibody

Background: Human growth hormone (GH) is a 22kDa monomeric protein produced and stored in somatotrophs in the anterior pituitary gland. GH is released from the pituitary into the bloodstream in a pulsatile manner under the regulatory control of hypothalamic somatostatin (SS) and GH-releasing factor (GHRF) [1]. The timing and frequency of GH release appears to be regulated by somatostatin, while the amplitude of GH release is determined by GHRF. A minor fraction (~1%) of GH in circulation exists in a smaller 2 kDa form [2]. GH has profound effects on tissue growth and metabolism, which is thought to be mediated through GH-dependent production of IGF-I and IGF-II, and their associated binding proteins. GH apparently stimulates IGF production after binding to specific cell surface receptors in the liver and, possibly, other tissues. Almost 5% of GH in circulation is bound to a high affinity GH binding protein (GHBP), which represents the extracellular domain of the GH receptor. Deficient GH secretion can occur in a number of clinical conditions [3]. However, evaluation of GH deficiency is complicated by the episodic nature of GH secretion and low circulating levels. A variety of physiologic and pharmacologic stimuli have been used to stimulate pituitary GH release during testing and failure to achieve a normal serum GH level in response to at least 2 GH stimulation or provocative tests is considered to be a diagnostic of GH deficiency [4]. The definition of a normal serum GH response is controversial, although published values generally range from 5 to 1 ng/mL. GH excess (or acromegaly) can be caused either by direct GH hypersecretion or GH excess secondary to GHRF hypersecretion. PRINCIPLES OF THE ASSAY This hGH enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre- coated with a monoclonal antibody specific to hGH. Standards or samples are then added to the microtiter plate wells and incubated. After wash all wells, hGH if present, will bind to the antibody pre-coated on the wells. In order to quantitatively determine the amount of hGH present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody specific to hGH is added to each well to this Human hGH ELISA Kit includes a set of calibration standards (6 standards). The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density (O.D.) versus GH concentration (ng/mL). The concentration of hGH in the samples is then determined by comparing the O.D. of the samples to the standard curve. S7.5(3) H-hGH