IGFBP5 ELISA Kit

Catalog No. ABIN625001

Human IGFBP5 ELISA Kit, Colorimetric assay for quantification of Human IGFBP5, that has been mentioned in 3+ publications.

Quick Overview for IGFBP5 ELISA Kit (ABIN625001)

Target: IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 2 ng/mL - 800 ng/mL

Application: ELISA

Sample Type: Plasma, Cell Culture Supernatant, Serum

Product Details IGFBP5 ELISA Kit

Minimum Detection Limit: 2 ng/mL

Purpose: Human IGFBP-5 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Analytical Method: Quantitative

Specificity: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, -2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Suggested Plasma Dilution: 2 fold

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

References for IGFBP5 ELISA Kit (ABIN625001)

Adali, Yorulmaz, Ozkanli, Ulasoglu, Bayraktar, Orhun, Colak, Tuncer: "Serum concentrations of insulin-like growth factor-binding protein 5 in Crohn's disease." in: World journal of gastroenterology, Vol. 19, Issue 47, pp. 9049-56, (2013) (PubMed).

Colak, Senates, Ozturk, Yilmaz, Zemheri, Yilmaz Enc, Ulasoglu, Aksaray, Bozbeyoglu, Kiziltas, Kurdas, Tuncer et al.: "Serum concentrations of human insulin-like growth factor-1 and levels of insulin-like growth factor-binding protein-5 in patients with nonalcoholic fatty liver disease: association with liver ..." in: European journal of gastroenterology & hepatology, Vol. 24, Issue 3, pp. 255-61, (2012) (PubMed).

Schalla, Hearn, Taylor, Eavenson, Valdiserri, Essien: "CDC's Model Performance Evaluation Program: assessment of the quality of laboratory performance for HIV-1 antibody testing." in: Public health reports (Washington, D.C. : 1974), Vol. 105, Issue 2, pp. 167-71, (1990) (PubMed).

Target Details for IGFBP5

Target: IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))

Alternative Name: IGFBP-5

Background:

Gene Symbols: IGFBP5, IBP5

Protein Name (Synonyms): Insulin-like growth factor-binding protein 5 (IBP-5) (IGF-binding protein 5) (IGFBP-5)

Gene ID: 3488

UniProt: P24593

Pathways: WNT Signaling, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding