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IL-6 ELISA Kit

This Human IL-6 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human IL-6.
Catalog No. ABIN625027

Quick Overview for IL-6 ELISA Kit (ABIN625027)

Target

See all IL-6 (IL6) ELISA Kits
IL-6 (IL6) (Interleukin 6 (IL6))

Reactivity

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  • 6
  • 4
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  • 3
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  • 3
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Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

3-1000 pg/mL

Application

ELISA

Sample Type

Tissue Lysate, Cell Lysate
  • Minimum Detection Limit

    3 pg/mL

    Purpose

    Human IL-6 ELISA Kit for cell and tissue lysate samples.

    Analytical Method

    Quantitative

    Specificity

    The antibody pair provided in this kit recognizes human IL-6.

    Sensitivity

    3 pg/mL

    Characteristics

    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    Components

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate

    Material not included

    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    • Cell lysate buffer
  • Sample Volume

    100 μL

    Plate

    Pre-coated

    Protocol

    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.

    Restrictions

    For Research Use only
  • Storage

    -20 °C

    Storage Comment

    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

    Expiry Date

    6 months
  • Target See all IL-6 (IL6) ELISA Kits

    IL-6 (IL6) (Interleukin 6 (IL6))

    Alternative Name

    IL-6

    Background

    Gene Names: IL6 IFNB2
    Protein names: Interleukin-6 (IL-6) (B-cell stimulatory factor 2) (BSF-2) (CTL differentiation factor) (CDF) (Hybridoma growth factor) (Interferon beta-2) (IFN-beta-2)

    Gene ID

    3569

    UniProt

    P05231

    Pathways

    TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome
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