Transferrin IQ-ELISA Kit

Catalog No. ABIN6386385

Product Details Transferrin IQ-ELISA Kit

Target: Transferrin (TF)

Reactivity: Human

Detection Method: qPCR

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human Immunoquantitative (PCR-Based) Transferrin ELISA Kit for cell culture supernatants, plasma, and serum samples.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Semi-Quantitative

Sensitivity: 0.1 ng/mL

Characteristics: IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.

Components:

• Transferrin Microplate (Item A): 96 well PCR plate coated with anti-Human Transferrin
• Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
• Standards (Item C): 2 vials of recombinant Human Transferrin
• Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
• Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
• Detection Affinity Reagent for Transferrin (Item F): 2 vials of a 4x concentrated solution of anti-Human Transferrin affinity reagent
• IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
• Primer Solution (Item I): 1.7 ml vial
• PCR Master Mix (Item J): 1.2 ml vial
• PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
• Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer

Material not included:

• Real-time PCR instrument, Bio-Rad recommended
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for data analysis
• Tubes to prepare standard or sample dilutions
• Heating block or water bath capable of 80^°C

Application Details

Application Notes: The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.

Comment:

The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.

Sample Volume: 25 µL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed
2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4^°C
3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
5. Add 15μL Primer solution 10μL of PCR master mix to each well
6. Run real-time PCR

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: Transferrin (TF)

Alternative Name: Transferrin (TF Products)

Synonyms: ltf IQ-ELISA Kit, pro1557 IQ-ELISA Kit, pro2086 IQ-ELISA Kit, mgc107777 IQ-ELISA Kit, LOC692564 IQ-ELISA Kit, TF IQ-ELISA Kit, LOC100144362 IQ-ELISA Kit, tf IQ-ELISA Kit, LTF IQ-ELISA Kit, TFEW IQ-ELISA Kit, conalbumin IQ-ELISA Kit, tf-b IQ-ELISA Kit, MGC64306 IQ-ELISA Kit, PRO1557 IQ-ELISA Kit, PRO2086 IQ-ELISA Kit, TFQTL1 IQ-ELISA Kit, AI266983 IQ-ELISA Kit, Cd176 IQ-ELISA Kit, HP IQ-ELISA Kit, Tf IQ-ELISA Kit, Tfn IQ-ELISA Kit, hpx IQ-ELISA Kit, Trf IQ-ELISA Kit, cb285 IQ-ELISA Kit, gavi IQ-ELISA Kit, id:ibd3238 IQ-ELISA Kit, id:ibd3525 IQ-ELISA Kit, sb:cb285 IQ-ELISA Kit, wu:fb57g06 IQ-ELISA Kit, wu:fb62h02 IQ-ELISA Kit, wu:fb63h10 IQ-ELISA Kit, wu:fb64h10 IQ-ELISA Kit, zgc:112154 IQ-ELISA Kit, 143958_at IQ-ELISA Kit, CG6186 IQ-ELISA Kit, Dmel\\CG6186 IQ-ELISA Kit, TSF1 IQ-ELISA Kit, anon-EST:Posey265 IQ-ELISA Kit, tsf1 IQ-ELISA Kit, Pro-TRH IQ-ELISA Kit, IL-5 IQ-ELISA Kit, STF I IQ-ELISA Kit, TRF1 IQ-ELISA Kit, sTF1 IQ-ELISA Kit, sTf IQ-ELISA Kit, tf1 IQ-ELISA Kit, transferrin IQ-ELISA Kit, serotransferrin IQ-ELISA Kit, transferrin (ovotransferrin) IQ-ELISA Kit, transferrin L homeolog IQ-ELISA Kit, melanotransferrin IQ-ELISA Kit, transferrin-a IQ-ELISA Kit, Transferrin 1 IQ-ELISA Kit, thyrotropin releasing hormone IQ-ELISA Kit, interleukin 5 IQ-ELISA Kit, TF IQ-ELISA Kit, LOC477072 IQ-ELISA Kit, tf IQ-ELISA Kit, Tf IQ-ELISA Kit, LOC100144362 IQ-ELISA Kit, tf.L IQ-ELISA Kit, LOC5575625 IQ-ELISA Kit, Trf IQ-ELISA Kit, tfa IQ-ELISA Kit, Tsf1 IQ-ELISA Kit, TRH IQ-ELISA Kit, IL5 IQ-ELISA Kit, LOC101085148 IQ-ELISA Kit, LOC100726872 IQ-ELISA Kit, trf IQ-ELISA Kit

Gene ID: 7018

UniProt: P02787

Pathways: Transition Metal Ion Homeostasis