TNFSF18 ELISA Kit

Catalog No. ABIN6719708

Product Details TNFSF18 ELISA Kit

Target: TNFSF18 (Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: Activation inducible TNF related ligand, Activation-inducible TNF-related ligand, AITRL, GITRL, Glucocorticoid induced TNF related ligand, Glucocorticoid induced TNFR related protein ligand, Glucocorticoid-induced TNF-related ligand, hGITRL, MGC138237, TL6, TNF18_HUMAN, TNFSF18, Tumor necrosis factor (ligand) superfamily member 18, Tumor necrosis factor ligand superfamily member 18

Background: Tumor necrosis factor ligand superfamily member 18, also known as AITRL or GITRL, is a protein that in humans is encoded by the TNFSF18 gene. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor(TNF) ligand family. It is mapped to 1q25.1. TNFSF18 is a ligand for receptor TNFRSF18/AITR/GITR. It has been shown to modulate T lymphocyte survival in peripheral tissues. This cytokine is also found to be expressed in endothelial cells, and is thought to be important for interaction between T lymphocytes and endothelial cells. TNFSF18-dependent modulation of tryptophan catabolism may represent an important mechanism of action of glucocorticoids, both physiologically and therapeutically.

Gene Name: TNFSF18

Production: Natural and recombinant mouse TNFSF18. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: T47-S173

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse TNFSF18 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse TNFSF18 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for TNFSF18

Target: TNFSF18 (Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18))

Alternative Name: TNFSF18 - GITRL (TNFSF18 Products)

Synonyms: Gitrl ELISA Kit, AITRL ELISA Kit, GITRL ELISA Kit, TL6 ELISA Kit, hGITRL ELISA Kit, TNLG2A ELISA Kit, tumor necrosis factor (ligand) superfamily, member 18 ELISA Kit, TNF superfamily member 18 ELISA Kit, Tnfsf18 ELISA Kit, TNFSF18 ELISA Kit

Gene ID: 240873

NCBI Accession: NP_899247

UniProt: Q7TS55