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IL12 ELISA Kit

This Mouse IL12 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse IL12.
Rockland
Catalog No. ABIN6719868
Supplier Product No.: koa0235

Quick Overview for IL12 ELISA Kit (ABIN6719868)

Target

See all IL12 ELISA Kits
IL12 (Interleukin 12 (IL12))

Reactivity

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Mouse

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

15.6 pg/mL - 1000 pg/mL

Application

ELISA

Sample Type

Cell Culture Supernatant, Serum
  • Minimum Detection Limit

    15.6 pg/mL

    Supplier Product No.

    koa0235

    Supplier

    Rockland

    Purpose

    Mouse IL-12 (p70) Sandwich ELISA Kit for Quantitative Detection

    Brand

    AccuSignal™

    Analytical Method

    Quantitative

    Specificity

    Production: Natural and recombinant mouse IL-12(P70). There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 1.5 pg/mL

    Components

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • Application Notes

    ELISA_Dilution: 15.6 pg/mL-1000 pg/mL

    Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse IL-12(P70) standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. It is recommended that each mouse IL-12(P70) standard solution and each sample be measured in duplicate.

    Comment

    Standard: Expression system for standard: SF21, Immunogen sequence: (M23-S419)+(R23-A215)

    Sample Volume

    100 μL

    Plate

    Pre-coated

    Restrictions

    For Research Use only
  • Storage

    4 °C,-20 °C

    Storage Comment

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    Expiry Date

    12 months
  • Target See all IL12 ELISA Kits

    IL12 (Interleukin 12 (IL12))

    Alternative Name

    IL-12(p70)

    Background

    Synonyms: CLMF, CLMF p35, Cytotoxic lymphocyte maturation factor 1, Cytotoxic lymphocyte maturation factor 1 p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, IL-12A, IL12A, IL12A_HUMAN, IL35 subunit, Interleukin 12 alpha chain, Interleukin 12 p35, Interleukin 12 p70, Interleukin-12 subunit alpha, Natural killer cell stimulatory factor 1, Natural killer cell stimulatory factor 1 35 kD subunit, NF cell stimulatory factor chain 1, NFSK, NK cell stimulatory factor chain 1, NKSF1, P35

    Background: Interleukin(IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins(p35 and p40) and is essential for the initiation of effective immune response. And the IL-12p70 is a heterodimer of p35 and p40 subunits, it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40(protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70. The standard product used in this kit is recombinant mouse IL-12 p70 comprising of a 193 amino acids p35 chain and a 313 amino acids p40 chain connected by a disulfide bond.

    Gene ID

    16159

    NCBI Accession

    NP_001152896

    UniProt

    F8WI71

    Pathways

    JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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