TNFSF14 ELISA Kit

Catalog No. ABIN6720322

Product Details TNFSF14 ELISA Kit

Target: TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: CD258, Delta transmembrane LIGHT, Herpes virus entry mediator ligand, Herpesvirus entry mediator A, Herpesvirus entry mediator ligand, HVEM L, HVEM-L, HVEML, Ligand for herpesvirus entry mediator, LTg, TNF14_HUMAN, Tnfsf14, Tumor necrosis factor (ligand) superfamily member 14, Tumor necrosis factor ligand superfamily member 14, Tumor necrosis factor receptor like 2, Tumor necrosis factor superfamily member LIGHT

Background: Tumor necrosis factor ligand superfamily member 14 is a protein that in humans is encoded by the TNFSF14 gene. TNFSF14 has also been designated as CD258, as well as LIGHT. It was mapped on chromosome 19p13.3. The protein encoded by this gene is a member of the tumor necrosis factor(TNF) ligand family. This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported.

Gene Name: TNFSF14

Production: Natural and recombinant human TNFSF14. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: D74-V240

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human TNFSF14 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma( heparin, EDTA) or saliva to each empty well. It is recommended that each human TNFSF14 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for TNFSF14

Target: TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))

Alternative Name: TNFSF14 - LIGHT (TNFSF14 Products)

Synonyms: TNFSF14 ELISA Kit, CD258 ELISA Kit, HVEML ELISA Kit, LIGHT ELISA Kit, LTg ELISA Kit, TR2 ELISA Kit, HVEM-L ELISA Kit, Ly113 ELISA Kit, TNF superfamily member 14 ELISA Kit, tumor necrosis factor (ligand) superfamily, member 14 ELISA Kit, TNFSF14 ELISA Kit, Tnfsf14 ELISA Kit

Gene ID: 8740

NCBI Accession: NP_003798

UniProt: O43557

Pathways: Cancer Immune Checkpoints