CD147 ELISA Kit

Catalog No. ABIN6720327

Supplier Product No.: koa0443

The Human CD147 ELISA Kit (ABIN6720327) is a Colorimetric ELISA Kit designed to quantify Human CD147.

Quick Overview for CD147 ELISA Kit (ABIN6720327)

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine

Product Details CD147 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Supplier Product No.: koa0443

Supplier: Rockland

Purpose: Human Emmprin Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: T25-H205

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 2 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Comment:

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human Emmprin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma( heparin, EDTA), saliva, urine or human milk to each empty well. It is recommended that each human Emmprin standard solution and each sample be measured in duplicate.

Assay Precision: Intra-Assay Precision (CV%): 7.6%, 7%, 7.2%
Inter-Assay Precision (CV%): 7.6%, 7.4%, 7.9%

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CD147

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Alternative Name: Emmprin

Background:

Background: Basigin is a member of the immunoglobulin superfamily that is also known as EMMPRIN, short for extracellular matrix metalloproteinase inducer, and recently has been designated CD147 (cluster of differentiation 147). It is a member of the immunoglobulin superfamily, with a structure related to the putative primordial form of the family. As members of the immunoglobulin superfamily play fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development, basigin is thought also to play a role in intercellular recognition (Miyauchi et al., 1991, Kanekura et al., 1991).This protein is a determinant for the Ok blood group system. Basigin is a type I integral membrane receptor that has many ligands, including the cyclophilin (CyP) proteins Cyp-A and CyP-B and certain integrins. It is expressed by many cell types, including epithelial cells, endothelial cells and leukocytes.

Gene Full Name: basigin (Ok blood group)

Gene ID: 682

NCBI Accession: NP_001719

UniProt: P35613

Pathways: S100 Proteins