CD147 ELISA Kit

Catalog No. ABIN921094

Human CD147 ELISA Kit, Colorimetric assay for quantification of Human CD147, that has been mentioned in 1 publication.

Quick Overview for CD147 ELISA Kit (ABIN921094)

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Binding Specificity: AA 25-205

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Urine, Milk

Product Details CD147 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: Sandwich Human EMMPRIN/CD147/BSG ELISA Kit to quantitate Human BSG in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: T25-H205

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 2 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 7.6%, 7%, 7.2%
Inter-Assay Precision (CV%): 7.6%, 7.4%, 7.9%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for CD147 ELISA Kit (ABIN921094)

Xie, Jiao, Chen, Xu, Li, Jiang, Zhang: "EMMPRIN (basigin/CD147) is involved in the morphogenesis of tooth germ in mouse molars." in: Histochemistry and cell biology, Vol. 133, Issue 5, pp. 585-94, (2010) (PubMed).

Target Details for CD147

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Alternative Name: BSG

Background:

Background: Basigin is a member of the immunoglobulin superfamily that is also known as EMMPRIN, short for extracellular matrix metalloproteinase inducer, and recently has been designated CD147 (cluster of differentiation 147). It is a member of the immunoglobulin superfamily, with a structure related to the putative primordial form of the family. As members of the immunoglobulin superfamily play fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development, basigin is thought also to play a role in intercellular recognition (Miyauchi et al., 1991, Kanekura et al., 1991).This protein is a determinant for the Ok blood group system. Basigin is a type I integral membrane receptor that has many ligands, including the cyclophilin (CyP) proteins Cyp-A and CyP-B and certain integrins. It is expressed by many cell types, including epithelial cells, endothelial cells and leukocytes.

Gene Full Name: basigin (Ok blood group)

Gene ID: 682

UniProt: P35613

Pathways: S100 Proteins