ERK2 ELISA Kit

Catalog No. ABIN6730481

Product Details ERK2 ELISA Kit

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human MAPK1/ERK2 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human MAPK1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for ERK2

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Alternative Name: MAPK1 (ERK2 (MAPK1 Products)

Synonyms: ERK-1 ELISA Kit, ERK1 ELISA Kit, ERT2 ELISA Kit, HS44KDAP ELISA Kit, HUMKER1A ELISA Kit, P44ERK1 ELISA Kit, P44MAPK ELISA Kit, PRKM3 ELISA Kit, p44-ERK1 ELISA Kit, p44-MAPK ELISA Kit, Erk-1 ELISA Kit, Erk1 ELISA Kit, Ert2 ELISA Kit, Esrk1 ELISA Kit, Mnk1 ELISA Kit, Mtap2k ELISA Kit, Prkm3 ELISA Kit, p44 ELISA Kit, p44erk1 ELISA Kit, p44mapk ELISA Kit, ERK ELISA Kit, ERK2 ELISA Kit, ERT1 ELISA Kit, MAPK2 ELISA Kit, P42MAPK ELISA Kit, PRKM1 ELISA Kit, PRKM2 ELISA Kit, p38 ELISA Kit, p40 ELISA Kit, p41 ELISA Kit, p41mapk ELISA Kit, 9030612K14Rik ELISA Kit, AA407128 ELISA Kit, AU018647 ELISA Kit, C78273 ELISA Kit, Erk2 ELISA Kit, Prkm1 ELISA Kit, p42mapk ELISA Kit, zERK2 ELISA Kit, MAPK1 ELISA Kit, Xp42 ELISA Kit, erk ELISA Kit, erk2 ELISA Kit, ert1 ELISA Kit, mapk ELISA Kit, mapk1b ELISA Kit, mapk2 ELISA Kit, mpk1 ELISA Kit, prkm1 ELISA Kit, prkm2 ELISA Kit, mapk1 ELISA Kit, mapk1a ELISA Kit, xp42 ELISA Kit, ATMPK1 ELISA Kit, F14N23.9 ELISA Kit, F14N23_9 ELISA Kit, MITOGEN-ACTIVATED PROTEIN KINASE ELISA Kit, mitogen-activated protein kinase 1 ELISA Kit, MNK1 ELISA Kit, ATMPK2 ELISA Kit, MITOGEN-ACTIVATED PROTEIN KINASE HOMOLOG 2 ELISA Kit, T30E16.13 ELISA Kit, T30E16_13 ELISA Kit, mitogen-activated protein kinase homolog 2 ELISA Kit, mitogen-activated protein kinase 3 ELISA Kit, mitogen-activated protein kinase 1 ELISA Kit, mitogen activated protein kinase 1 ELISA Kit, mitogen-activated protein kinase 1 L homeolog ELISA Kit, mitogen-activated protein kinase 1 S homeolog ELISA Kit, mitogen activated protein kinase 3 ELISA Kit, mitogen-activated protein kinase homolog 2 ELISA Kit, MAPK3 ELISA Kit, Mapk3 ELISA Kit, MAPK1 ELISA Kit, Mapk1 ELISA Kit, mapk1 ELISA Kit, mapk1.L ELISA Kit, mapk1.S ELISA Kit, PfMAP1 ELISA Kit, MPK1 ELISA Kit, PVX_084965 ELISA Kit, LOC9328617 ELISA Kit, Tsp_01601 ELISA Kit, MPK2 ELISA Kit

UniProt: P28482

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins