MEK2 ELISA Kit

Catalog No. ABIN6730501

Product Details MEK2 ELISA Kit

Target: MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human MEK2/MAP2K2 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human MEK2.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for MEK2

Target: MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

Alternative Name: MEK2 (MAP2K2 (MAP2K2 Products)

Synonyms: CFC4 ELISA Kit, MAPKK2 ELISA Kit, MEK2 ELISA Kit, MKK2 ELISA Kit, PRKMK2 ELISA Kit, AA589381 ELISA Kit, MK2 ELISA Kit, Prkmk2 ELISA Kit, ATMKK2 ELISA Kit, F27B13.50 ELISA Kit, F27B13_50 ELISA Kit, MAP KINASE KINASE 1 ELISA Kit, MAP kinase kinase 2 ELISA Kit, MK1 ELISA Kit, map2k2 ELISA Kit, wu:fa56c06 ELISA Kit, zgc:123171 ELISA Kit, zgc:172250 ELISA Kit, mitogen-activated protein kinase kinase 2 ELISA Kit, mitogen activated protein kinase kinase 2 ELISA Kit, MAP kinase kinase 2 ELISA Kit, mitogen-activated protein kinase kinase 2a ELISA Kit, mitogen-activated protein kinase kinase 2b ELISA Kit, MAP2K2 ELISA Kit, Map2k2 ELISA Kit, MKK2 ELISA Kit, map2k2a ELISA Kit, map2k2b ELISA Kit

UniProt: P36507

Pathways: MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling