JNK ELISA Kit (Mitogen-Activated Protein Kinase 8)

Details for Product MAPK8 ELISA Kit No. ABIN6730536
Antigen
  • JNK
  • JNK-46
  • JNK1
  • JNK1A2
  • JNK21B1/2
  • PRKM8
  • SAPK1
  • SAPK1c
  • AI849689
  • Prkm8
  • BSK
  • BSK/DJNK
  • Bsk
  • CG5680
  • D-JNK
  • D-junk
  • DBSK/JNK
  • DJNK
  • DJNK/bsk
  • Dmel\\CG5680
  • JNK/SAPK
  • Jnk
  • Junk
  • SAPKa
  • c-Jun
  • dJNK
  • jnk
  • T10F20.15
  • jnk1
  • sapk1
  • mapk8
  • zgc:112379
  • JNKb
  • mapk8b
  • mitogen-activated protein kinase 8
  • Stress-activated protein kinase jnk-1
  • basket
  • Protein kinase superfamily protein
  • mitogen-activated protein kinase 8 L homeolog
  • mitogen-activated protein kinase 8b
  • mitogen-activated protein kinase 8B-like
  • MAPK8
  • jnk-1
  • Mapk8
  • bsk
  • ATMPK8
  • mapk8.L
  • mapk8b
  • LOC109094263
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
12
10
10
2
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Options
Purpose Mouse JNK1 ELISA Kit.
Sample Type Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This ELISA antibody pair recognizes Mouse JNK1.
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Plasmids, Primers & others Plasmids, Primers & others JNK products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name JNK1 (MAPK8 ELISA Kit Abstract)
UniProt A3KBF5
Pathways MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor
Application Notes Optimal working dilution should be determined by the investigator.
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months
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