JNK ELISA Kit

Catalog No. ABIN6730536

Product Details JNK ELISA Kit

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse JNK1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse JNK1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for JNK

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Alternative Name: JNK1 (MAPK8 Products)

Synonyms: JNK ELISA Kit, JNK-46 ELISA Kit, JNK1 ELISA Kit, JNK1A2 ELISA Kit, JNK21B1/2 ELISA Kit, PRKM8 ELISA Kit, SAPK1 ELISA Kit, SAPK1c ELISA Kit, AI849689 ELISA Kit, Prkm8 ELISA Kit, BSK ELISA Kit, BSK/DJNK ELISA Kit, Bsk ELISA Kit, CG5680 ELISA Kit, D-JNK ELISA Kit, D-junk ELISA Kit, DBSK/JNK ELISA Kit, DJNK ELISA Kit, DJNK/bsk ELISA Kit, Dmel\\CG5680 ELISA Kit, JNK/SAPK ELISA Kit, Jnk ELISA Kit, Junk ELISA Kit, SAPKa ELISA Kit, c-Jun ELISA Kit, dJNK ELISA Kit, jnk ELISA Kit, T10F20.15 ELISA Kit, jnk1 ELISA Kit, sapk1 ELISA Kit, mapk8 ELISA Kit, zgc:112379 ELISA Kit, JNKb ELISA Kit, mapk8b ELISA Kit, mitogen-activated protein kinase 8 ELISA Kit, Stress-activated protein kinase jnk-1 ELISA Kit, basket ELISA Kit, Protein kinase superfamily protein ELISA Kit, mitogen-activated protein kinase 8 L homeolog ELISA Kit, mitogen-activated protein kinase 8b ELISA Kit, mitogen-activated protein kinase 8B-like ELISA Kit, MAPK8 ELISA Kit, jnk-1 ELISA Kit, Mapk8 ELISA Kit, bsk ELISA Kit, ATMPK8 ELISA Kit, mapk8.L ELISA Kit, mapk8b ELISA Kit, LOC109094263 ELISA Kit

UniProt: A3KBF5

Pathways: MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins