SOCS6 ELISA Kit

Catalog No. ABIN6951237

Product Details SOCS6 ELISA Kit

Target: SOCS6 (Suppressor of Cytokine Signaling 6 (SOCS6))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human socs6 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for SOCS6

Target: SOCS6 (Suppressor of Cytokine Signaling 6 (SOCS6))

Alternative Name: socs6 (SOCS6 Products)

Synonyms: CIS-4 ELISA Kit, CIS4 ELISA Kit, HSPC060 ELISA Kit, SOCS-4 ELISA Kit, SOCS-6 ELISA Kit, SOCS4 ELISA Kit, SSI4 ELISA Kit, STAI4 ELISA Kit, STATI4 ELISA Kit, socs6 ELISA Kit, MGC81477 ELISA Kit, SOCS6 ELISA Kit, 1500012M23Rik ELISA Kit, 5830401B18Rik ELISA Kit, AI447482 ELISA Kit, Cis4 ELISA Kit, Cish4 ELISA Kit, Socs4 ELISA Kit, socs6l ELISA Kit, zgc:64107 ELISA Kit, wu:fb77f06 ELISA Kit, suppressor of cytokine signaling 6 ELISA Kit, suppressor of cytokine signaling 6 L homeolog ELISA Kit, suppressor of cytokine signaling ELISA Kit, suppressor of cytokine signaling 6b ELISA Kit, SOCS6 ELISA Kit, Socs6 ELISA Kit, socs6.L ELISA Kit, socs6 ELISA Kit, socs6b ELISA Kit

Gene ID: 9306

UniProt: O14544

Pathways: JAK-STAT Signaling, Carbohydrate Homeostasis