THEM2 ELISA Kit

Catalog No. ABIN6951297

Product Details THEM2 ELISA Kit

Target: THEM2 (ACOT13) (Acyl-CoA Thioesterase 13 (ACOT13))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human ACOT13 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for THEM2

Target: THEM2 (ACOT13) (Acyl-CoA Thioesterase 13 (ACOT13))

Alternative Name: ACOT13 (ACOT13 Products)

Synonyms: them2 ELISA Kit, MGC89869 ELISA Kit, si:dkey-218n20.4 ELISA Kit, THEM2 ELISA Kit, DKFZp468B074 ELISA Kit, PNAS-27 ELISA Kit, 0610006O17Rik ELISA Kit, Them2 ELISA Kit, acyl-CoA thioesterase 13 ELISA Kit, acyl-CoA thioesterase 13 S homeolog ELISA Kit, thioesterase superfamily member 2 ELISA Kit, Thioesterase superfamily protein ELISA Kit, acot13 ELISA Kit, ACOT13 ELISA Kit, acot13.S ELISA Kit, LOC733047 ELISA Kit, si605090c10 ELISA Kit, Acot13 ELISA Kit

Gene ID: 55856

UniProt: Q9NPJ3