BRCA1 ELISA Kit
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- Target See all BRCA1 ELISA Kits
- BRCA1 (Breast Cancer 1 (BRCA1))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- Human BRCA1 ELISA Kit.
- Sample Type
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product BRCA1 ELISA Kit
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Plate
- Pre-coated
- Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Expiry Date
- 6 months
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- Target See all BRCA1 ELISA Kits
- BRCA1 (Breast Cancer 1 (BRCA1))
- Alternative Name
- BRCA1 (BRCA1 Products)
- Synonyms
- BRCAI ELISA Kit, BRCC1 ELISA Kit, BROVCA1 ELISA Kit, IRIS ELISA Kit, PNCA4 ELISA Kit, PPP1R53 ELISA Kit, PSCP ELISA Kit, RNF53 ELISA Kit, iris ELISA Kit, pscp ELISA Kit, brcai ELISA Kit, brcc1 ELISA Kit, rnf53 ELISA Kit, brca1 ELISA Kit, BRCA1 ELISA Kit, LOC100224649 ELISA Kit, BRCA1, DNA repair associated ELISA Kit, breast cancer 1 ELISA Kit, breast cancer 1, early onset ELISA Kit, breast cancer 1 L homeolog ELISA Kit, BRCA1 ELISA Kit, brca1 ELISA Kit, LOC755086 ELISA Kit, Brca1 ELISA Kit, brca1.L ELISA Kit
- Gene ID
- 672
- UniProt
- P38398
- Pathways
- Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus
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