CDKN2D ELISA Kit

Catalog No. ABIN6951426

Product Details CDKN2D ELISA Kit

Target: CDKN2D (Cyclin-Dependent Kinase Inhibitor 2D (p19, Inhibits CDK4) (CDKN2D))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human CDKN2D ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for CDKN2D

Target: CDKN2D (Cyclin-Dependent Kinase Inhibitor 2D (p19, Inhibits CDK4) (CDKN2D))

Alternative Name: CDKN2D (CDKN2D Products)

Synonyms: ink4d ELISA Kit, p19 ELISA Kit, p19-ink4d ELISA Kit, CDKN2D ELISA Kit, ank2 ELISA Kit, INK4D ELISA Kit, p19-INK4D ELISA Kit, INK4d ELISA Kit, p19INK4d ELISA Kit, cyclin-dependent kinase inhibitor 2D S homeolog ELISA Kit, cyclin dependent kinase inhibitor 2D ELISA Kit, cyclin-dependent kinase inhibitor 2D ELISA Kit, Cyclin-dependent kinase 4 inhibitor D ELISA Kit, cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4) ELISA Kit, similar to cyclin-dependent kinase inhibitor 2D ELISA Kit, cdkn2d.S ELISA Kit, CDKN2D ELISA Kit, cdkn2d ELISA Kit, cdn2d ELISA Kit, Cdkn2d ELISA Kit

Gene ID: 1032

UniProt: P55273

Pathways: Cell Division Cycle, Sensory Perception of Sound, Mitotic G1-G1/S Phases, Negative Regulation of intrinsic apoptotic Signaling