GADD45A ELISA Kit

Catalog No. ABIN6951442

Product Details GADD45A ELISA Kit

Target: GADD45A (Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human GADD45A ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for GADD45A

Target: GADD45A (Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A))

Alternative Name: GADD45A (GADD45A Products)

Synonyms: MGC53491 ELISA Kit, zgc:91795 ELISA Kit, gadd45a ELISA Kit, GADD45A ELISA Kit, ddit1 ELISA Kit, gadd45 ELISA Kit, GADD45 ELISA Kit, AA545191 ELISA Kit, Ddit1 ELISA Kit, DDIT1 ELISA Kit, Gadd45 ELISA Kit, growth arrest and DNA damage inducible alpha L homeolog ELISA Kit, growth arrest and DNA-damage-inducible, alpha, b ELISA Kit, growth arrest and DNA damage inducible alpha ELISA Kit, growth arrest and DNA damage-inducible protein 45 ELISA Kit, growth arrest and DNA-damage-inducible 45 alpha ELISA Kit, growth arrest and DNA-damage-inducible, alpha ELISA Kit, gadd45a.L ELISA Kit, gadd45ab ELISA Kit, GADD45A ELISA Kit, gadd45a ELISA Kit, GADD45 ELISA Kit, Gadd45a ELISA Kit

Gene ID: 1647

UniProt: P24522

Pathways: p53 Signaling, Cell Division Cycle